Composite

Part:BBa_K2957047:Design

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-21)


CMV-2xGal4 (Gal4 repressible promoter), mKate2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1392
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1392
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1392
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used Modular Cloning Golden Gate Type II Assembly, so we had to consider how parts came together. See more on the MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT>.


Source

Parts of this part were ordered from IDT/Twist and then assembled with other parts (from iGEM/parts in our parts collection/from the Weiss lab) into this plasmid.

References